Lipid Analysis

The field of lipidomics has been driven by development in mass spectrometry and associated separation techniques. SLING currently maintains 11 mass spectrometry instruments dedicated to lipid analysis.

We have developed a range of ‘non-targeted’ and ‘targeted’ methods, mainly based on liquid chromatography (UPLC) and mass spectrometry, as well as gas chromatography and supercritical fluid chromatography.

Non-targeted lipidomics relies on high accuracy and resolution mass measurements using Time-of-Flight and Orbitrap technologies to capture a comprehensive view of a tissue’s lipidome, or compare lipidome-scale changes in  given conditions.

Our targeted methods have been optimised for routine quantitative analyses of various classes of lipids (neutral lipids, phosphoglycerolipids, sphingolipids, fatty acids, sterols and derivatives) in a variety a of tissues (plants, bacteria, yeast, animal models, human). We use chromatographic separation and tailored MRM transitions lists on triple quadrupole instruments.