The field of lipidomics has been driven by development in mass spectrometry and associated separation techniques. SLING currently maintains 11 mass spectrometry instruments dedicated to lipid analysis.
We have developed a range of ‘non-targeted’ and ‘targeted’ methods, mainly based on liquid chromatography (UPLC) and mass spectrometry, as well as gas chromatography and supercritical fluid chromatography.
Non-targeted lipidomics relies on high accuracy and resolution mass measurements using Time-of-Flight and Orbitrap technologies to capture a comprehensive view of a tissue’s lipidome, or compare lipidome-scale changes in given conditions.
Our targeted methods have been optimised for routine quantitative analyses of various classes of lipids (neutral lipids, phosphoglycerolipids, sphingolipids, fatty acids, sterols and derivatives) in a variety a of tissues (plants, bacteria, yeast, animal models, human). We use chromatographic separation and tailored MRM transitions lists on triple quadrupole instruments.